FP7 NANOREG
A common European approach to the regulatory testing of nanomaterials
This project has received funding from the European Union's FP7 for research, technological development and demonstration under grant agreement no 310584 NANoREG
In NANoREG over 85 institutional partners from EU member states, associated states, the Republic of Korea and Brazil collaborated in developing reliable, reproducible and relevant methods for testing and assessing the effects of nanomaterials on human health and environment in a regulatory context.
The duration of the project was 48 months; the end date was 28 February 2017. The budget was approximately fifty million euro. Ten million was provided by the EU (FP7 programme) and forty million by member states, regions, partners and other sources.
As -probably the first- nanosafety project, all the information and knowledge generated under the umbrella of the NANoREG project has been made available for other parties directly after the ending of the project. The NANoREG Results Repository serves as a central point of access to all the results of the project by providing links to all relevant documents, datasets and other information of interest.
A large part of the NANoREG experimental nano-EHS data is available via the NANoREG - eNanoMapper database
It contains data gathered via TNO Data Entry Tool (snapshot as of 22.03.2017). The coming months more data will be added.
For NANoREG partners It was mandatory to carry out the experiments with NANoREG core materials. For all these core materials a complete state-of-the-art physicochemical characterization was done considering key end-points in the OECD WPMN sponsorship program.
Dispersion SOPs and minimum requirements for characterization were established and laid down in a Guidance Document thus supporting reliability and comparability of data. Experiments had to be performed in accordance with the Guidance Document. During the course of the project, those fundamental requirements were further refined. The data in the data base can be further analyzed by various tools
- NM-100
- NM-101
- NM-102
- NM-103
- NM-104
- NM-105
- NM-110
- NM-111
- NM-112
- NM-113
- NM-200
- NM-201
- NM-202
- NM-203
- NM-204
- NM-400
- NM-401
- NM-402
- NM-403
nanoreg
MTS CELL VIABILITY ASSAY Impedance adherent cells ALAMAR BLUE Impedance flow cytometry NRU Spectrophotometric Method viability COMET RT-PCR MICRONUCLEUS ASSAY COMET FPG CBMN assay Manual scoring LUCIFER YELLOW Intestinal barrier integrity intestinal barrier integrity SEM-EDX TEM ELISA Annexin V_PI (FACS) Griess Reagent LUMINEX® Reaction Oxygen Species Detection DLS Batch Dispersion Quality supplier WAXS VSSA SUPPLIER TGA Filtration ICP-MS DAPI Hoechst 33342 CELL COUNTER Nuclear Intensity Nuclear area CFE Mitochondrial Membrane Potential dye Lysosomal dye Ki-67 apoptosis cell cycle cleaved caspase-3 Genotoxicity ATM phospho S1981 H2AX phospho S139 p53 phospho s15 Annexin V (Apoptosis) / PI (Necrosis) Nanoparticle tracking Analysis SEM SOP-Toxicity Test with Daphnia magna (without NOM) Weighing Optical Microscope FACS analysis Colorimetric assay Keylab cell counter CCK8 assay Pig-A gene Swiss roll HE-Pycnometer in vitro oral mucosa 3D model Confocal microscopy TEM/STEM SAXS OECD TG487 MTT assay ELISA MULTIPLEX method barrier test May grunwald giemsa coloration of BAL cells cytospin biochemistry automate ICP-MS automatic count WAXD sp_ICP-MS Aerosol Characterisation XRD Dustiness JRCNM01001a ISO 10872:2010 Water QualityDetermination of the toxic effects of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans OECD TG 202 Acute Toxicity OECD TG 201 ATR-FTIR HAADF-STEM CHN-analysis commercial kits manual count ICP-OES Potentiometry PSEUDOKIRCHNERIELLA SUBCAPITATA CLS Colimetry Propidium iodide Manual count /100 cells vibrofluidization Vortex Shaker LDH DCFH2-DA EDX TRYPAN BLUE PrestoBlue protocol A13261nanoreg
CACO-2 THP-1 A549 BEAS-2B 3T3 HepG2 GF SaOs CAKI-1 CALU-3 Hep3B U937 RAW 264.7 TK6 NHBE Peritoneal primary macrophages PERIPHERAL BLOOD - LEUKOCYTES BONE MARROW CELLS Liver cells SPLEEN CELLS Epithelial cells from colon Epithelial cells from duodenum Kidney cells OVARY CELLS Testicular cells epididymis-spermatozoa MLN PERIPHERAL BLOOD A549:THP1 brain cell V79 Lung cells BAL HMDM